• Company : Shenzhen Lvshiyuan Biotechnology Co., Ltd
  • Region : Guangdong , China
  • Category : Health & Medical > Examination & Testing Instrument
  • Specification : 1.Sensitivity:0.05ppb 2.ISO9001:2008 3.Spec.:96 wells/kit 4.MOQ:1 kit
  • Quantity : 100
  • Price : 250
  • Contact Person : Bella Zou|||Overseas sales
  • Telephone :
  • Fax :
  • Mobile phone :
  • Website : www.lsybt.com
  • Email : [email protected]
  • Instant Messenger : Skype: Bellazou3
  • Update : Dec/19/2014
  • Product Details

    Tetracyclines(TCs) ELISA Test Kit Catalog No. LSY-10006 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tetracyclines in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.05 ppb Incubation Temperature: 25? Incubation Time: 30min—15min Detection limit: Tissue (chicken): Tetracyclines 0.4ppb Minocycline 0.4ppb RoliTetracyclines 0.4ppb Aureomycin 0.4ppb Demethylchlor Tetracyclines 1.2ppb Terramycin 0.8ppb Doxycycline 1ppb Pork, Honey Tetracyclines 0.5ppb Minocycline 0.5ppb RoliTetracyclines 0.5ppb Aureomycin 0.5ppb Demethylchlor Tetracyclines 1.5ppb Terramycin 1ppb Doxycycline 1.2ppb Cross-reaction rate: Tetracyclines 100% Minocycline 125% RoliTetracyclines 110% Aureomycin 100% Demethylchlor Tetracyclines 35% Terramycin 58% Doxycycline 45% Recovery rate: Chicken, Pork, honey 85±20% 3. Components 1 Micro-well strips 12 strips with 8 removable wells each 2 Standard Concentrate (81ppb) 1ml black cap 3 High-concentration standard (100ppb) 1ml black cap 4 Enzyme conjugate 7ml white cap 5 Antibody working solution 7ml white cap 6 Substrate A 7ml white cap 7 Substrate B 7ml black cap 8 Stop solution 7ml yellow cap 9 20× concentrated washing buffer 15ml white cap 10 10× sample extract 50ml * 2 transparent cap 11 Standard redissolving solution 15ml *2 blue cap