- Company : Shenzhen Lvshiyuan Biotechnology Co., Ltd
- Region : Guangdong , China
- Category : Health & Medical > Biological Products
- Specification : 1.Spec.:96 wells/kit 2.Sample performance: tissue,honey 3.MOQ: 1 kit 4.ISO9001:2008
- Quantity : 100
- Price : 300
- Contact Person : Bella Zou|||Overseas sales
- Telephone :
- Fax :
- Mobile phone :
- Website : www.lsybt.com
- Email : [email protected]
- Instant Messenger : Skype: Bellazou3
- Update : Sep/17/2014
Product Details
Nitrofuran (AOZ) ELISA Test Kit Catalog No. LSY-10002 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of AOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AOZ in it. This value is compared to the standard curve and the AOZ concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.01ppb Incubation Temperature: 25? Incubation Time: 30min?15min Cross-reaction rate AOZ 100% AMOZ <0.1% AHD <0.1% SEM <0.1% Recovery rate Tissue 95±25% Honey 85±23% 3. Components 1? Micro-well strips: 12 strips with 8 removable wells each 2? 6× standard solution (1 mL each): 0ppb, 0.01ppb, 0.03ppb, 0.09ppb, 0.27ppb and 0.81ppb 3? Enzyme conjugate (7 mL) red cap 4? Antibody working solution (7 mL) blue cap 5? Substrate A solution (7 mL) white cap 6? Substrate B solution (7 mL) black cap 7? Stop solution (7 mL) yellow cap 8? 20× concentrated washing buffer (40 mL) white cap 9? 2× concentrated redissolving solution (50 mL) transparent cap 10? 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap
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