- Company : Shenzhen Lvshiyuan Biotechnology Co., Ltd
- Region : Guangdong , China
- Category : Health & Medical > Biological Products
- Specification : 1.Sensitivity: 0.5ppb 2.ISO9001:2008 3.MOQ:1 kit 4.Spec.: 96 wells/kit
- Quantity : 100
- Price : 350
- Contact Person : Bella Zou|||Overseas sales
- Telephone :
- Fax :
- Mobile phone :
- Website : www.lsybt.com
- Email : [email protected]
- Instant Messenger : Skype: Bellazou3
- Update : Dec/19/2014
Product Details
Neomycin ELISA test Kit Catalog No. LSY-10005 1. Principle This test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Neomycin residues in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Neomycin in it. This value is compared to the standard curve and the Neomycin concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.5 ppb Detection limit Tissue 10 ppb Honey 10 ppb Milk, milk powder 10 ppb Recovery rate Chicken, pork, honey, fish, shrimp 85±15% Liver (chicken or porcine) 70±10% Milk, milk powder ………………………………………………………….. 85±10% Cross-reaction rate Neomycin 100% Streptomycin <0.1% Gentamicin <0.1% Dihydrostreptomycin <0.1% Kanamycin <0.1% Tobramycin <0.1% Sisomicin <0.1% 3. Components 1? Micro-well strips: 12 strips with 8 removable wells each 2? 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb 3? Enzyme conjugate (7 mL) red cap 4? Antibody concentrated solution (7 mL) blue cap 5? Substrate A solution (7 mL) white cap 6? Substrate B solution (7 mL) black cap 7? Stop solution (7 mL) yellow cap 8? 20× concentrated washing buffer (40 mL) white cap 9? 2× concentrated redissolving solution (50 mL) transparent cap
