- Company : Shenzhen Lvshiyuan Biotechnology Co., Ltd
- Region : Guangdong , China
- Category : Health & Medical > Biological Products
- Specification : 1.Sensitivity:1ppb 2.Packing: 96 wells/kit 3.ISO9001:2008 4.MOQ:1 kit
- Quantity : 100
- Price : 300
- Contact Person : Bella Zou|||Overseas sales
- Telephone :
- Fax :
- Mobile phone :
- Website : www.lsybt.com
- Email : [email protected]
- Instant Messenger : Skype: Bellazou3
- Update : Dec/19/2014
Product Details
Melamine ELISA Test Kit Catalog No. LSY-10026 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Melamine in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Melamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for anti-Melamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the Melamine concentration in the sample. This value is compared to the standard curve and the Melamine concentration is subsequently obtained. 2. Technical specifications Sensitivity: 1 ppb Incubator temperature: 25? Incubator time: 30min?15min Detection limit: Milk powder 20 ppb Milk 16 ppb Milk/Milk powder (Method two) 1 ppb Tissue (chicken, pork, duck, fish, shrimp and liver) 2 ppb Feed 100 ppb Egg 20 ppb Serum 4 ppb Cross-reaction rate: Melamine 100% Cyanuric acid 60% Triazine <1% Diamino atrazine <1% Recovery rate: Milk/Milk powder 90±19% Tissue 85±20% Feed 85±20% Egg 80±20% 3. Components 1? Micro-well strips: 12 strips with 8 removable wells each 2? 6× standard solution (1 mL each): 0 ppb,1 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb 3? Enzyme conjugate (7 mL) red cap 4? Antibody working solution (7 mL) blue cap 5? Substrate A solution (7 mL) white cap 6? Substrate B solution (7 mL) black cap 7? Stop solution (7 mL) yellow cap 8? 20× concentrated washing buffer (40 mL) white cap 9? 2× concentrated redissolving solution (50 mL) transparent cap
