- Company : Shenzhen Biokau Sci-tech Co.,ltd
- Region : Guangdong , China
- Category : Chemicals > Chemical Reagents
- Quantity : 1
- Contact Person : Samara Duan|||Sales Manager
- Telephone :
- Mobile phone :
- Website : www.biokau.com
- Email : [email protected]
- Instant Messenger : skype:samara (foodsafety...)
- Update : Apr/07/2015
Product Details
1. Usage This kit is used to detect Avian Marek's disease virus antibody in chicken serum, to assess antibody condition by Avian Marek's disease vaccine in chicken farm and assist diagnosis of serological infected chicken. 2. Principle The Avian Marek's disease antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm). 3. Reagents 1) Antigen coated microplate................................................ 1 piece (96 wells) 2) Enzyme conjugate.................................................... 1 bottle (12 mL/bottle) 3) 10×concentrated washing buffer…………………………1 bottle (50 mL/bottle) 4) Substrate A /B........................................................ 1 tube each (6 mL/tube) 5) Sample diluent……………………………………………. 1 bottle (50 mL/bottle) 6) Stop solution................................................................. 1 tube (6 mL/tube) 7) Negative control serum................................................ 1 tube (800?L /tube) 8) Positive control serum................................................. 1 tube (800?L /tube) 9) Serum diluent plate……………………………………………………..…..1 piece 10) Adhesive film……………………………………………………………….2 pieces 11) Instruction……………………………………………………………………1 piece 4. Materials required but not provided 1) Micropipettors and disposable tips: 0.5?L~10?L?10?L~100?L?100?L
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