Swine Foot and Mouth Disease antibody ELISA kit

• Company : Shenzhen Biokau Sci-tech Co.,ltd
• Region : Guangdong , China
• Category : Chemicals > Chemical Reagents
• Quantity : 1
• Contact Person : Samara Duan|||Sales Manager
• Telephone :
• Mobile phone :
• Website : www.biokau.com
• Email : [email protected]
• Instant Messenger : skype:samara(foodsafety...)
• Update : Apr/07/2015

• Product Details

  1. Introduction The BIOKAU® FMD-Ab ELISA is developed to detect the O type antibodies of Porcine Foot and Mouth Disease in porcine serum samples and assess the immunity conditions against FMD in the pig farms. 2. Principle The kit is based on an indirect enzymatic immunoassay.The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm). 3. The kit components 1 FMD coated microplate................................................. 2 pieces (192 wells) 2 FMD Negative control serum (green lid).................................. 1 tube (1 mL) 3 FMD Positive control serum (red lid)....................................... 1 tube (1 mL) 4 FMD Enzyme conjugate (yellow lid)...................................... 1 bottle (22 mL) 5 20×concentrated washing buffer (white lid)........................... 1 bottle (30 mL) 6 Substrate A (purple lid)/B (yellow lid)......................................... (12 mL/each) 7 Stop solution (blue lid)......................................................... 1 bottle (12 mL) 8 Sample diluent (transparent lid)............................................ 1 bottle (50 mL) 4. Precautions and warnings for users 1. Read the use instructions carefully. 2. Bring all reagents to room temperature prior to use. 3. Do not use components after expiration dates and do not mix components from different lots. 4. If the dry bag is red, don’t use. 5. Don’t pollute any components. 6. Reseal the plates after use and avoid to wet. 7. Do not pipette by mouth 8. Use gloves and Be careful with reagents 9. Clean the discarded reagents 5. Materials Required But N

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