- Company : Shenzhen Biokau Sci-tech Co.,ltd
- Region : Guangdong , China
- Category : Health & Medical > Biological Products
- Quantity : 1
- Contact Person : Samara Duan|||Sales Manager
- Telephone :
- Mobile phone :
- Website : www.biokau.com
- Email : [email protected]
- Instant Messenger : skype:Samara Duan(foodsafety...)
- Update : Jul/07/2015
Product Details
1. Usage The BIOKAU® porcine circovirous ELISA Test kit is used for detection of porcine circovirus antibodies in the serum; assessment of immunity conditions against porcine circovirus in the pig farm and investigation of the epidemiology of the porcine circovirus. 2. Principle The BIOKAU® PCV2 ELISA kit is based on an indirect enzymatic immunoassay(Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm). 3. The kit components 1 Antigen coated microtiter plate......................................... 2 pieces (96 wells) 2 Negative (green lid)/positive (red lid)serum............. 1 tube each (1.5 mL/tube) 3 Anti-pig IgG-HRP conjugate (yellow lid)....................... 1 bottle (22 mL/bottle) 4 20×concentrated washing buffer (white lid).................. 1 bottle (50 mL/bottle) 5 Substrate A (purple lid)/B (yellow lid)................... 1 bottle each (12 mL/bottle) 6 Stop solution(blue lid)................................................ 1 bottle (12 mL/bottle) 7 Sample diluent solution(transparent lid)....................... 1 bottle (50 mL/bottle) 8 Adhesive Foil................................................................................. 6 pieces 9 Instruction....................................................................................... 1 piece 4. Material Required But not Provided 1 Microplate Reader(wave length: 450/630 nm). 2 Microplate Washer. 3 Micropipettes, adjustable. 4 Constant temperature box or incubator. 5 Oscillator. 5. Sample requirement 1 The samples are porcine serum, which should be collected with no bacteria
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