Porcine Japanese B Encephalitis Virus Antibody ELISA Test Kit

• Company : Shenzhen Biokau Sci-tech Co.,ltd
• Region : Guangdong , China
• Category : Chemicals > Chemical Reagents
• Quantity : 1
• Contact Person : Samara Duan|||Sales Manager
• Telephone :
• Mobile phone :
• Website : www.biokau.com
• Email : [email protected]
• Instant Messenger : skype:samara(foodsafety...)
• Update : Apr/07/2015

• Product Details

  1. Usage The Porcine Japanese B Encephalitis Virus Antibody ELISA Test Kit (JEV Ab) is used for the detection of the porcine encephalitis virus antibodies in swine serum; assessment the immunity conditions against porcine encephalitis virus in the pig farms and investigation of the epidemiology of the porcine encephalitis virus. 2. Principle The kit is based on an indirect enzymatic immunoassay(Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm). 3. The kit components 1 Antigen coated microtiter plate......................................... 2 pieces (96 wells) 2 Negative (green lid)/positive (red lid)serum................ 1 tube each (1 mL/tube) 3 Anti-pig IgG-HRP conjugate (yellow lid)....................... 1 bottle (22 mL/bottle) 4 20×concentrated washing buffer (white lid).................. 1 bottle (30 mL/bottle) 5 Substrate A (purple lid)/B (black lid)..................... 1 bottle each (12 mL/bottle) 6 Stop solution(blue lid)................................................ 1 bottle (12 mL/bottle) 7 Sample diluent solution(transparent lid)....................... 1 bottle (50 mL/bottle) 4. Precautions and warnings for users 1. Read the use instructions carefully. 2. Bring all reagents to room temperature prior to use. 3. Do not use components after expiration dates and do not mix components from different lots. 4. If the dry bag is red, don’t use. 5. Don’t pollute any components. 6. Reseal the plates after use and avoid to wet. 7. Do not pipette by mouth 8. Use gloves and Be careful with reagents 9. Clean the discarded reagents 5.

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