- Company : Shenzhen Biokau Sci-tech Co.,ltd
- Region : Guangdong , China
- Category : Chemicals > Chemical Reagents
- Quantity : 1
- Contact Person : Samara Duan|||Sales Manager
- Telephone :
- Mobile phone :
- Website : www.biokau.com
- Email : [email protected]
- Instant Messenger : skype:samara(foodsafety...)
- Update : Apr/07/2015
Product Details
1. Summary This kit use Canine rabies virus solid phase antigen to detect specific antibody against Canine rabies virus antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and used repeatedly. 2. Reagents and contents 1. 8× Coated microtiter strips: ready to use 2. 2× Conjugate stock solution: ready to use 3. 2× Conjugate diluent: ready to use 4. 1× PBST powder: ready to use 5. 1× Substrate A solution: ready to use 6. 1× Substrate A solution: ready to use 7. 1× Stopping solution: ready to use ?Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor.? 8. 1× Positive control: ready to use 9. 1× Negative control: ready to use 10. 1× Instruction sheet 3. Test procedure 1. Conjugate solution preparation: add one conjugate stock solution into one conjugate diluent , mix thoroughly; 2. Solution preparation: Dissolve each bag PBST powder with 500 ml distilled water, 3. Adding samples and controls: Leave well A1 for reagent blank. Pipette controls and samples as follows: Add two drops diluent into each well, then add 5 ?l serum sample into each well separately, mix evenly. Synchronously add critical control and negative control each 100 ?l into each two wells without diluent and add two drops diluent as the blank. incubate in dark at 37 ? for 20 minutes, then discard the liquid of the well, add 1 drop washing solution into each well and fill with distilled water promptly, incubate for 30 seconds, then discard the liquid, continue perform another 4 washing cycles as above, then discard the liquid, flap to dry with the absorbent paper. 4. Adding conjugate solution: Dispense 2 drops into all wells (except blank well) and incubate at 37? for 20 minutes. Discard the contents of the wells and wash 5 times as describe
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