- Company : Shenzhen Biokau Sci-tech Co.,ltd
- Region : Guangdong , China
- Category : Chemicals > Chemical Reagents
- Quantity : 1
- Contact Person : Samara Duan|||Sales Manager
- Telephone :
- Mobile phone :
- Website : www.biokau.com
- Email : [email protected]
- Instant Messenger : skype:samara(foodsafety...)
- Update : Apr/07/2015
Product Details
Details 1. Usage The BIOKAU® Swine Foot and Mouth Disease Distinguishing ELISA Test kit, or called FMD-3ABC ELISA kit is used for detection the non-structural 3ABC-protein of swine foot and mouth disease (FMD-3ABC) antibodies in the serum, and differentiation naturally infected from vaccined swine. 2. Brief Introduction The BIOKAU® FMD-3ABC test kit is made of the antigen coated microplate, enzyme conjugation (HRP goat-anti-pig IgG) and other reagents, which applies the indirect ELISA principle to test the antibodies against FMD-3ABC in the serum. 3. Reagents 1 3ABC Antigen coated microplate....................................... 2 pieces (96 wells) 2 Negative (green lid)/positive (red lid) serum................ 1 tube each (1 mL/tube) 3 3 ABC Enzyme conjugate (yellow lid)........................... 1 bottle (22 mL/bottle) 4 20× concentrated washing solution (white lid)............... 1 bottle (30 mL/bottle) 5 Substrate A (purple lid)/B (yellow lid).................... 1 bottle each (12 mL/bottle) 6 Stop solution (blue lid)................................................ 1 bottle (12 mL/bottle) 7 Sample diluent solution (transparent lid)....................... 1 bottle (50 mL/bottle) 4. Material Required But not Provided Micropipettes, Distilled water, Constant temperature box or incubator, microplate-reader, Absorbent paper. 5. Warning and Procautions 1. All reagents should be adjusted to the room temperature before using the test kit. 2. Never mix up reagents from test kits in different batch number. 3. Prevent the microplate to get damp. 4. Keep dry of remaining microplates after use 6. Preparation Dilute 20× concentrated washing solution at 1: 19 with distilled water. 7. Test Procedure 1. Take out the pre-coated microplate. Dilute the sample at 1:39 with the sample diluent and add to the wells, 100 ?L each. Set 2 wells for negative control serum and 2 wells f
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