- Company : Shenzhen Biokau Sci-tech Co.,ltd
- Region : Guangdong , China
- Category : Health & Medical > Veterinary Medicine
- Quantity : 1
- Contact Person : Samara Duan|||Sales Manager
- Telephone :
- Mobile phone :
- Website : www.biokau.com
- Email : [email protected]
- Instant Messenger : skype:Samara Duan(foodsafety...)
- Update : Jul/06/2015
Product Details
1. Principle The BIOKAU® Malachite green ELISA test kit is based on the competitive enzyme immunoassay. The coupling antigen is pre-coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Malachite green in it. This value is compared to the standard curve and the content of Malachite green residues is subsequently obtained. 2. Technical specifications Sensitivity: 0.025 ppb Detection limit Shrimp, Fish..................................................................................... 0.1 ppb Water............................................................................................... 0.1 ppb Recovery rate Shrimp, Fish.................................................................................... 75±10% Cross-reaction rate Malachite green................................................................................... 100% Leucomalachite green........................................................................... 0.1% Gentian violet ....................................................................................... 95% Leucocrystal violet................................................................................ 0.1% 3. Components 1? Micro-well strips: 12 strips with 8 removable wells each 2? Standard solution (1 mL)............................................................... 100 ppb 3? Enzyme conjugate (12 mL)............................................................. red cap 4? Antibody working solution (7 mL).................................................... red cap 5? Substrate solution A (7 mL).....................................
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